A Study On The Protective Immunity - 928 Words

In this study, the CRPV/HLA-A2.1 transgenic rabbit model was used to assess the protective immunity generated by DNA vaccines delivered using the gene gun, the tattoo gun or the microneedle system. The focus was to determine whether the tattoo gun and the microneedle delivery systems were useful DNA vaccination alternatives to the gene gun. Our laboratory has successfully utilized the gene gun in our DNA vaccination studies for both protective and therapeutic purposes (17,34). However, the gene gun system is costly, and we are constantly looking for alternatives to back up our vaccine development program. Tattoo gun has been reported to deliver a DNA vaccine successfully in both mice and non-human primates (21-23). Compared with the gene gun and microneedle, tattoo gun delivery is more cost effective but more invasive which generates trauma and scars the vaccinated tissues. Microneedle delivery has achieved comparable protection in mice with twice the dose of gene gun delivery (24 ). However, no studies have been attempted in rabbits for DNA vaccination with either a tattoo gun or a microneedle. In the current study, we compared the gene gun with the tattoo gun as well as the tattoo gun with microneedle DNA vaccine delivery using our well-studied DNA epitope vaccines and our unique HLA-A2.1 transgenic rabbit model (39). Read out was tumor outgrowth rates and tumor size. We demonstrated that these two novel DNA delivery systems can be used for DNA vaccination in the rabbitShow MoreRelatedInteractions Between Fungi And DC900 Words   |  4 Pagesand DC: How DC can be used to develop vaccines? Like mentioned previously, DC initiates activation of different types of cells based on type of molecules/antigens at site of infection. Hence different forms of fungi initiate different mechanisms of immunity. When DC pulsed with fungus are introduced into mice, they can initiate specific immune response against that antigen by activating CD4+ Th cells. The analysis of antigen specific proliferation and cytokine production from CD4+ Th cells was doneRead MoreA Research Analysis Of MERS-COV Mice912 Words   |  4 PagesAn experimental study done on Six-to eight-week-old specific pathogen-free female BALB/c mice to test if DNA vaccine encoding MERS-COV S protein can induce immunity against MERS-COV infection. The study generated 3 recombinant plasmids expressing MERS-CoV spike protein: pcDNA3.1-S, pcDNA3.1-SDCD, and pcDNA3.1-S1 which dissolved into Phosphate-buffered saline (PBS) with final concertation of 1  µg /  µl. Mice were divide randomly to experimental group which injected intramuscularly with 100  µg recombinantRead MoreRapid Diagnostic Tests In Rwanda Case Study1129 Words   |  5 Pagesmalaria transmission Combined malaria histidine rich protein 2 (HRP2)/ Plasmodium lactate dehydrogenase (pLDH) rapid diagnostic tests (RDTs) are often used to determine whether persons with fever should be treated with anti-malarials. The observation study carried out in Rwanda on 9226 from April 2014- April 2015 to compare the sensitivity of RDTs based on HRP2 and the Plasmodium lactate dehydrogenase (pLDH) in diagnosing malaria using microscopy and molecular test(PCR) to see if there is associationRead More##syl Lipid A ( GLA ) Adjuvants : Associated Antigen Portrayed In Fig1857 Words   |  4 Pagesassociated antigen (Rv1813) as shown in Fig1. The protein has been identified to recognize human T cells [11] and has the capacity to stimulate Th1 immune responses known to confer protective immunity that is required for an effective TB vaccine [12]. However, as is generally known, recombinant proteins are by themselves poorly immunogenic and need an adjuvant to stimulate adaptive immune responses. The Glucopyranosyl Lipid A (GLA) adjuvant is a synthetic Toll-like Receptor (TLR) 4 that is formulatedRead MoreVaccinations : Should They Be Mandatory?1331 Words   |  6 Pagesmicroorganisms or toxins or of antibodies or lymphocytes† that is administered predominantly to thwart virus replication of a particular disease (Vaccine). It acts as a stimulant for the immune system of a human body which assists in developing adaptive immunity to a specific pathogen. Shockingly, vaccines themselves are created using components found in the virus or the bacteria itself (Offit). As of present, no federal vaccination laws exist in the United States of America although all states require childrenRead MoreHemoglobin Case Study940 Words   |  4 PagesThis study was aimed to assess the hemoglobin level, and associated factors to the development of neonatal infection in Neonatal Intensive Care Unit (NICU). In our study of 256 neonates, the most prevalent diagnosis on admission was Hypoxic-Ischemic Encephalopathy (HIE) of new born (43.00%) followed by prematurity (29.70%). Birth weight (with mean=1889g,), gestational age (mean= 34.2 weeks), hospital stay (mean=12days) and hemoglobin level (mean=14 ±2 g/dl) had greater odds for development of neonatalRead MoreWay to Stronger, Healthier and a More Immune India1833 Words   |  7 Pagesimmunization - Colostrum: We are responsible for immunity of the babies to start developing right from birth itself. It is the babys right to drink the mothers first milk expressed which is called colostrum. It has large amount of main immune factor Immunoglobulin A(IgA) which forms a protective layer on the mucous membranes in the babys intestines, nose and throat. This protective layer protects the babies from invading germs. The protective layer formed in intestinal tract prevent babies fromRead MoreBreastfeeding : The Natural Source Of Nutrition For Babies1175 Words   |  5 Pagesfluid that a newborn needs in the early days after birth, as well as substances to protect the baby diseases. Compared to regular breast milk, colostrum is yellow in color, and very dense. Its color and thickness are a result of it being higher in protective nutrients and antibodies. (HHS- Office on Women’s Health, 4). Also, colostrum is higher in protein, slightly lower in sugar, extremely low in fat. A mother’s body will produce colostrum for many days after child birth, until milk production increasesRead MoreEssay On Natural Immunity Against Malaria1574 Words   |  7 PagesNatural Immunity against malaria: Natural immunity against asexual stage parasites develops with repetitive exposure to malaria parasites and thus forms the basis of clinical immunity against malaria (Baird, Jones et al. 1991, Day and Marsh 1991, Trape, Rogier et al. 1994, Baird 1995, Baird 1998, Hviid 2005). Though clinical immunity to malaria has been shown to develop in individuals, it does not prevent reinfection due to several factors including the complex life-cycle of the parasite and insufficiencyRead MoreThe Epidemic Of West Nile1599 Words   |  7 Pageschallenge were used in the 1999-2000 studies. These challenge models result in about 90% of the nonvaccinated control horses developing viremia, but only 10% demonstrated clinical disease. The intrathecal challenge model is the newest version. 70 to 90% of nonvaccinated control horses become visemic and 90 to 100% develop grave signs of encephalomyelitis. West Nile virus vaccines are licensed either as 1) an aid in protection against viremia or 2) protective against viremia and clinical disease